Cairo From

Course Objectives:

• Upon Completion of the Course, Participants will be able to:
• The aim is to provide first hand information to the participants on optimal use of gas chromatograph (GC).
• A special emphasis has been given on the demonstration of applica­tions, maintenance and trouble­shooting of GC.
• Imparting participants’ fundamen­tal knowledge of Gas Chromatog­raphy as a technique.
• To understand GC maintenance methods as a routine checks.
• Knowledge of accessories and con­sumables required for its opera­tion.
• To familiarize participants to the techniques for achieving gas chro­matography analysis, qualitative and quantitative methods, cause and effect diagrams and standard curve.

Who should attend?:

• All who works in any laboratory, i.e. clinical, industry, gas, petroleum, refinery, phar­macy, etc.
• Laboratory manager and supervisor
• Laboratory engineers and chemists
• Laboratory analysts and technicians
• Instrument engineers/technicians

Course Outline:

Day 1:

• Theory Parameters:
• Plate height(H),
• Retention Time,
• Capacity Fac­tor,
• Selectivity Factor,
• Column Efficiency,
• Column Resolution.
• Sampling And Sample Handling Iso 17025:
• Requirements for Gases,
• Liquid and Solid,
• Sample Sampling Methods,
• Sample Registra­tion and Storage,
• Sample Disposal,
• Max Holding Time of Sample,
• Sample Contamination.

• Sample Preparation And Analyte Manipulation Technique:
• Solid-phase extraction,
• Static head space technique,
• Soxhlet extraction,
• Microwave-assisted extraction,
• Super critical fluid extraction

Day 2:

• Derivatization Method:
• Silyl,
• Alkyl,
• Acylation and Esterfication Methods
• Types Of GC:
• Normal GC,
• High Speed GC and Portable GC
• Gc Separation And Retention Process:
• Dispersive Interac­tions,
• Dipole-Dipole Interactions,
• Dipole-Induced Dipole Interactions
• Carrier Gas:
• Van Deemter equation,
• Optimal Practical Gas Velocity(OPGV),
• Average Linear Velocity,
• Flow Rate,
• Carrier Gas Selection,
• Pressure Regulator,
• Gas Purification
• Sample Injection System:
• Sample Introduction Technique,
• Manual Injection,
• Solvent Flush Injection,
• Septa,
• Inlet Liner,
• Split/Split less Inlets System,
• Spit Ratio,
• Purge Delay Time,
• Cool On-Column Inlets,
• Programmed Temperature Vaporization,
• Headspace Technique,
• Injection Problems,
• Hot Vaporizing Injector

Day 3:

• Column Configurations:
• Stationary Phase of Packed and Capillary Column,
• Packed Columns,
• Temperature programming,
• Gas-Liquid and Gas- Solid Columns,
• Polarity and Selectivity,
• Column Coating
• Detectors:
• Ideal Characteristics,
• Column Position,
• Gases,
• Flame Ionization Detector,
• Thermal Conductivity Detector,
• Flame Photometric Detector,
• Nitrogen Phosphorous Detector,
• Photo Ionization Detector,
• Electron Capture Detector,
• Mass Spectrometry(Fragmentation and identify technique with software training),
• Chemiluminescent Detector,
• Mass Analyzer – Time of Flight,
• Ion Trap and Quadrupole Technique.
• Maintenance And Installation Methods:
• Clean and Condition Septa,
• Cleaning Injector Liners,
• Silylating Liners,
• Column Conditioning,
• Installation Fused Silica Capillary Columns,
• Column Placement in the oven,
• Column Installation,
• Leak Detection,
• Bleed Test,
• FidJet Cleaning Procedure,
• TCD Clean Detector Cell,
• Cleaning Of ECD,
• FPD Mainte­nance,
• Cleaning MS and Change The Filament

Day 4:

• Instrument Problems And Troubleshooting:
• Approaches to Solve GC Problems,
• Troubleshooting Tests,
• Band Broadening,
• Broadening Initial Peak Band widths,
• Retention Gap Sampling,
• Sampling By Solute Fo­cusing,
• Retention Gaps Tube,
• Baseline Deviation,
• Noisy Baseline,
• Spikes in Baseline,
• Peak Shape Problems,
• Flat Top Peaks,
• Split Peaks,
• Negative Peaks,
• Retention Changes,
• Ghost Peak,
• Causes and Prevention of Column Damage,
• Column Contamination,
• Needle Discrimination,
• Change in Detec­tors Sensitivity,
• Difficulty in Lighting Fid Flame,
• Loss of Detectors Linear Range,
• Leaks in MS,
• Excessive Noise or High Background in MS
• Practical Demonstration:
• Introduction and familiarization with GC,
• Preparation of sample test,
• Preparation of standards,
• Setting the methods program,
• Inject the sample and standards,
• Print the chromatograms,
• Check all GC components and how to do the preventive maintenance

Day 5:

• Quality Control And Validation Method:
• Specificity,
• Linear­ity,
• Selectivity,
• Accuracy,
• Precision,
• Bias,
• Linearity,
• Range,
• Limit of De­tection,
• Limit Of Quantification,
• Robustness,
• Ruggedness,
• Stability
• Quantitative Methods:
• Interpolated Graph Calibration by Using External and Internal Standards,
• Standard Addition Method,
• Calibration Methods,
• Correlation Coefficient,
• Outliers Test of Repeated Measure­ments,
• Outliers In Calibration Curve,
• Errors In Quantitative Analysis,
• Distribution of Errors,
• Confidence Limit,
• Limit of Detection,
• Repeatabil­ity,
• Reproducibility,
• Optimization Methods
• Course summary .
• Course fees: 3600 US$ PER EACH PARTICIPANT
• 10% - For 2 nominations
• 15% - For 3 nominations
• 20% - For 4 nominations
• 25% - For 5 nominations
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